A common request that we often get at Visikol is if we can immunolabel microtissues encapsulated or grown in Matrigel; one of the first 3D cell culture options commercially available and a well know protein-based scaffold.
A critical aspect of high content microscopy is processing large numbers of images in an automated fashion. In this blog post, Dr. Tom Villani highlights how to create an ImageJ macro for batch-processing of large image sets acquired from high content microscopy.
With growing interest in leveraging in vitro models to reduce costs, increase throughput, and minimize ethical concerns throughout the early stages of drug discovery and development, microplates utilized in in vitro assays have become increasingly specialized. But, with the wide variety of microplate types on the market today, plate choice can be a daunting decision when setting up any new assay. Many considerations need to be taken into account such as culture approach (if any), throughput requirements, and analytical needs. Below we summarize a few of these considerations.
Immunofluorescence is a powerful tool that we utilize at Visikol to image whole tissues and 3D cell culture models. The imaging of large tissues can provide powerful quantitative insight into the sample of interest like protein colocalization, spatial information about cell viability, and penetration depth of test compounds.
In this blog post Dr. Tom Villani discusses loading and processing 3D confocal image stacks and performing volume measurements with ImageJ. This is crucial for processing data from tissues imaged in 3D with confocal or light sheet microscopy through the use of tissue clearing.