Dr. Jay Deng from Ecor Bio has applied the Visikol HISTO methodology to visualize several EnCor Bio immunolabels in 3D. Below you can see the penetration depth for each immunolabel as well as some z-stacks obtained from tissue using confocal microscopy with our standard immunolabeling protocol. The penetration of immunolabels will depend upon the specific type of immunolabel as well as what tissue you are looking at and how it has been prepared. Reach out to us about further extending the penetration of immunolabels into tissues. 

Antibodies EnCor Cat # Mouse Brain Rat Brain Penetration Depth
chicken glial fibrilaral acdic protein CPCA-GFAP >600 um (rat)
chicken calbindin CPCA-Calb > 550 um (rat/mouse)
chicken calretinin CPCA-Calretinin > 600 um (rat)
chicken myelin basic protein CPCA-MBP < 300 um (rat)
chicken neurofilament-heavy chain CPCA-NFH < 300 um (rat)
chicken vimentin CPCA-VIM < 150 um (rat/mouse)
mouse Fox3/NeuN MCA-1B7 > 600 um (rat)
mouse glial fibrilaral acidic protein MCA-5C10 < 300 um (rat)
mouse myelin basic protein MCA-7G7 < 400 um (rat)
mouse neurofilament-heavy chain MCA-AH1 < 50 um (rat)
mouse vimentin MCA-2D1 < 430 um (rat)
rabbit cFos RPCA-cFos > 500 um (rat)
rabbit Fox3/NeuN RPCA-Fox3 > 600 (rat)
rabbit methy CpG-Binding Protein 2 RPCA-MeCP2 > 550 um (rat/mouse)
rabbit neurofilament-medium chain RCPA-NFM < 200 um (rat/mouse)
Mouse Brain 20X, 5C10 (red‐546 )/chMBP (green‐488)/Dapi (blue‐405), 600 um

Mouse Brain 20X, 5C10 (red‐546 )/chMBP (green‐488)/Dapi (blue‐405), 600 um

Rat Brain 20X, 5C10 (red‐546 )/chMBP (green‐488)/Dapi (blue‐405), 600 um

Rat Brain 20X, 5C10 (red‐546 )/chMBP (green‐488)/Dapi (blue‐405), 600 um

Rat Brain 20X, Rab‐cFos (red‐546 )/mo NFH‐AH1 (green‐488)/Dapi (blue‐405), 50 um

Rat Brain 20X, Rab‐cFos (red‐546 )/mo NFH‐AH1 (green‐488)/Dapi (blue‐405), 50 um