Visikol® HISTO™ tissue clearing shown to be reversible and non-destructive

Recently, the Visikol team demonstrated the reversibility of clearing with Visikol® HISTO™.  Why is this important? Now you can image important bio-markers in 3D, then you can reverse the tissue and embed in paraffin for H&E sectioning, and since you have already seen the 3D structure, your histopathologist knows exactly which sections to analyze! This is a powerful addition to the bio-imaging workflow, as 3D images of protein expression can be correlated directly to histopathology. Therefore, Visikol® HISTO™ can be used within the normal tissue processing workflow, whereas other techniques need to be done in parallel to histopathology.

In the last five years, tremendous progress has been made in developing and optimizing tissue clearing and labeling techniques for the 3D visualization of tissues. While these techniques have yielded impressive 3D images and have gotten significant attention, they have not been widely adopted into mainstream research. Through customer interviews and extensive market research, we have determined that there are two main hurdles in the way of this technologies adoption:

1)      Difficulty of Tissue Clearing Techniques: The main reason that we hear from researchers for why they have not tried tissue clearing is that they simply do not know how. Current clearing techniques like CLARITY require numerous steps, special equipment, and can take a long period of time. The knowledge gap for adopting these techniques is very high and most researchers do not want to spend resources and time on a technique that can destroy their tissues. This is the main problem we are trying to solve with Visikol® HISTO™ by providing easy-to-use and rapid tools.

2)      Validation: While stunning 3D images of whole tissues like the brain are incredibly impressive, it is challenging to correlate these findings to histopathological results. The optimum clearing technique would leave tissue intact for all subsequent histological analysis to allow for cross-validation of data extracted from 3D. Because current tissue clearing techniques irreversibly damage tissues through swelling, shrinking or lipid removal, these approaches are challenging to validate.

To learn more about the reversibility of Visikol® HISTO™: click here.


 H&E Slide of Mouse Heart Following Visikol® HISTO™ Reversal. 

H&E Slide of Mouse Heart Following Visikol® HISTO™ Reversal. 

Michael Johnson