Visikol® HISTO™ Protocol
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Figure 1. Cleared mouse brain tissue, stained with DAPI, single section 500 µm into brain tissue; imaged on Leica TCS SPII Confocal Microscope.
Figure 2. Cleared mouse brain tissue, image taken at 500 um depth into brain tissue, stained with NeuroTrace Yellow (ThermoFisher Scientific, cat #N21481); Imaged with Leica TCS SPII confocal microscope.
Figure 3. HepG2 micro-tissue, cleared with Visikol; labeled for EGFR using rabbit anti-EGFR with anti-rabbit AlexaFluor488-conjugated secondary antibody; z-projection of 10 µm interval optical sections.
Renier, N., Wu, Z., Simon, D. J., Yang, J., Ariel, P., & Tessier-Lavigne, M. (2014). iDISCO: a simple, rapid method to immunolabel large tissue samples for volume imaging. Cell, 159(4), 896–910. http://doi.org/10.1016/j.cell.2014.10.010