Below is a list of antibodies which have been validated for use in IMC using Maxpar® OnDemand Antibodies. If your antibody is not on this list, it can be easily conjugated using a MaxPar custom conjugation kit to build your panel of interest. We routinely work with clients to develop custom panels to ensure that we best meet their multiplex tissue imaging needs.
| Target | Tissue Type | Reported Reactivity |
|---|---|---|
| CD98 | FFPE | Human |
| Alpha-Smooth Muscle Actin | FFPE, Frozen | Human, Mouse |
| Anti-B-actin | FFPE | Cross, Human, Mouse |
| Arginase-1 | FFPE | Cross, Human |
| aSMA (1A4) | FFPE, Frozen | Human, Mouse |
| B7-H4 | FFPE | Human |
| BCL-2 | FFPE | Human, Mouse |
| BCL-6 | FFPE | Human, Mouse |
| Beta Tubulin | FFPE | Cross, Human, Mouse |
| Beta-Catenin | FFPE | Human, Mouse, Rat |
| BRCA1 | FFPE | Human, Mouse |
| Caspase-3 cleaved | FFPE | Cross, Human |
| CD107a/LAMP1 | FFPE | Human |
| CD11b | FFPE | Human, Mouse |
| CD11c | Frozen | Cross, Human |
| CD127 | FFPE | Cross, Human, Mouse |
| CD134 | FFPE | Human, Mouse |
| CD14 | FFPE | Human, Mouse |
| CD15 | FFPE, Frozen | Human |
| CD16 | FFPE, Frozen | Human |
| CD16 | FFPE, Frozen | Human, Rat |
| CD163 | FFPE, Frozen | Human |
| CD163 | FFPE, Frozen | Human |
| CD19 | FFPE | Cross, Human |
| CD194/CCR4 | FFPE | Human |
| CD196 (CCR6) | Frozen | Cross, Human |
| CD20 | Frozen | Cross, Human |
| CD20 | FFPE | Human |
| CD223/LAG3 | FFPE | Human |
| CD227/Mucin 1 | FFPE | Human |
| CD235ab | FFPE | Human |
| CD25 | FFPE | Human |
| CD27 | Frozen | Human |
| CD274/PD-L1 | FFPE, Frozen | Human |
| CD276/B7-H3 | FFPE | Human |
| CD278/ICOS | FFPE | Human |
| CD279/PD-1 | Frozen | Cross, Human |
| CD28 | Frozen | Human |
| CD3 | FFPE | Human, Mouse |
| CD31 | Frozen | Cross, Human |
| CD31 | FFPE | Human, Mouse, Rat |
| CD326 (EpCAM) | FFPE, Frozen | Human |
| CD366/TIM | FFPE | Human, Mouse |
| CD38 | Frozen | Cross, Human |
| CD4 | Frozen | Cross, Human |
| CD4 | FFPE | Mouse |
| CD44 | FFPE, Frozen | Human, Mouse |
| CD45 | FFPE, Frozen | Human |
| CD45 | FFPE | Mouse |
| CD45R/B220 | FFPE | Human, Mouse |
| CD45RA | FFPE, Frozen | Cross, Human |
| CD45RO | FFPE, Frozen | Cross, Human |
| CD45RO | FFPE, Frozen | Human, Monkey, Rhesus, Chimpanzee |
| CD57 | FFPE | Human |
| CD63 | FFPE | Human |
| CD66a | FFPE | Human |
| CD66b | FFPE | Human |
| CD68 | FFPE, Frozen | Human |
| CD68 | FFPE, Frozen | Human |
| CD73 | FFPE | Human |
| CD74 | FFPE | Human, Mouse |
| CD8 | FFPE | Mouse |
| CD86 | Frozen | Cross, Human |
| CD8A | Frozen | Cross, Human |
| CD8a | FFPE | Human |
| C-Myc p67 | FFPE | Human |
| Collagen I | FFPE, Frozen | Human, Mouse |
| Cytokeratin | FFPE | Cross, Human, Mouse |
| E-Cadherin | FFPE, Frozen | Cross, Human, Mouse |
| EGFR | FFPE | Cross, Human |
| EpCAM | FFPE | Human, Mouse, Rat |
| F4/80 | FFPE | Mouse |
| Fibronectin | FFPE | Human, Mouse, Rat |
| FoxP3 | FFPE | Mouse, Bovine, Dog, Cat, Pig, Rat |
| Granzyme B | FFPE | Human, Mouse, Rat |
| Histone H3 | FFPE | Human, Mouse, Rat, |
| HLA-ABC | Frozen | Cross, Human |
| HLA-DR | FFPE, Frozen | Human, Rhesus |
| IDO | FFPE | Human |
| IgM | Frozen | Cross, Human |
| iNOS | FFPE | Human, Mouse |
| Keratin 8/18 | FFPE | Human |
| Ki-67 | FFPE, Frozen | Cross, Human, Mouse |
| Ly-6G | FFPE | Mouse |
| Met | FFPE | Human, Mouse |
| MHC Class II | FFPE | Mouse |
| p53 | FFPE | Human |
| Pan-CK | FFPE, Frozen | Cross, Human, Mouse |
| Pan-Actin | FFPE | Human, Mouse |
| Pan-Keratin | FFPE | Human |
| PD-1 | FFPE | Human |
| PD-L1 | FFPE | Human |
| PD-L2 | FFPE | Human |
| Perforin | Frozen | Cross, Human |
| pERK1/2 [T202/Y204] | FFPE | Human, Mouse, Rat |
| pH2AX [S139] | FFPE | Human, Mouse |
| pHistone H3 [Ser28] | FFPE | Cross, Human |
| Phospho- | FFPE | Cross, Human, Mouse |
| Phospho-4EBP1 | FFPE | Cross, Human, Mouse |
| Phospho-CREB [Ser133] | FFPE | Cross, Human, Mouse |
| Phosphop44/42 MAPK | FFPE | Human, Mouse |
| Phospho-Stat3 | FFPE | Human |
| p-NF-kBp65 | FFPE | Human, Mouse |
| pS6 | FFPE | Human, Mouse |
| SOX-9 | FFPE | Cross, Human, Mouse |
| Syk | FFPE | Human |
| T-bet | FFPE | Human |
| VEGF | FFPE | Human |
| Vimentin | FFPE, Frozen | Human, Mouse, Rat, Rabbit, Bovine, Canine, Hamster, Monkey, Cross |
| Vista | FFPE | Human |
17-plex IMC Panel
We also have several pre-built panels, such as the 17-plex IMC panel described below.
The 17-plex panel for IMC combines 3 panels together to assess the complexities of
the immune landscape within the tumor microenvironment.
Human Tissue Architecture Panel
Enables staining of tissue architecture,
including stroma, epithelial tissue,
and vasculature.
- Alpha-smooth muscle actin
- Collage type I
- E-cadherin
- Histone H3
- Vimentin
Human Tumor Infiltrating Lymphocytes Panel
Enables staining of epithelial cells; phenotyping of T cells (helper, cytotoxic, memory, and regulatory), B cells, and monocytes/macrophages; and identification of potential CD68+ tumor cells.
- CD20
- CD3
- CD4
- CD45RO
- CD68
- CD8a
- FoxP3
- Pan-keratin
Human Immune
Activation Panel
Allows for the phenotyping of
activated T cells.
- Granzyme B
- Ki-67
- PD-1
- PD-L1