Visikol® HISTO™ Starter Kit

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DIY Kit Box.png
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Visikol® HISTO™ Starter Kit

559.99

With the Visikol HISTO tissue clearing approach you are now able to rapidly and easily image tissues in 3D without damaging the underlying cellular morphology. The Visikol HISTO approach has been designed to pair with immunolabeling and is reversible so that 3D histology can be followed up with traditional 2D histology. The Visikol HISTO tissue clearing approach is comprised of two different reagents called Visikol HISTO-1 and Visikol HISTO-2 whereas Visikol HISTO-1 is used first and Visikol HISTO-2 used second.

In our Starter Kit we have combined 30 mL of Visikol HISTO-1 and 30 mL of Visikol HISTO-2 with the five pre-treatment solutions required to process tissue for immunolabeling.  All you need to do is add your immunolabels and nuclear stains. This kit comes with enough material to process four whole mouse brains (approx. 2 grams of tissue). If you want to test out Visikol HISTO before you buy it or have any questions email us at: info@visikol.com

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In our Visikol® HISTO™ Starter Kit we have combined 30 mL of Visikol HISTO-1 and 30 mL of Visikol HISTO-2 with the buffer solutions required to process tissue for immunolabeling. All you need to do is add your immunolabels and nuclear stains. This kit comes with enough material to process four whole mouse brains (approx. 2 grams of tissue). If you want to test out Visikol HISTO before you buy it or have any questions email us at: info@visikol.com

Included in the Starter Kit

Not included in this kit

In the Starter Kit we provide everything except for antibodies and commonly available laboratory reagents:

  • Primary and secondary antibodies 
  • Nuclear stain – Any common nuclear stain can be used, e.g. DAPI, Hoeschst 33342 , SYTOX stains (ThermoFisher Cat# S7020) or TO-PRO-3 (ThermoFisher Cat#T3605
  • Methanol or ethanol – Approx. 500 mL required
  • Phosphate buffered saline (PBS) – Approx. 1.5 liters
  • PBS with 0.2% - 1% Triton™ X-100 – approximately 100 mL 
  • Container for sample – We suggest scintillation vials, falcon tubes or eppendorf tubes 
  • 4% Paraformaldehyde solution (PFA), freshly prepared – Approx. 30 mL

Storage

Upon receipt, after opening, all the reagents except Visikol HISTO-1 and Visikol HISTO-2 should be stored in the refrigerator at 4°C, and handled carefully after opening, as they are aqueous buffers and could be subject to contamination after opening. 

Information