Optical Clearing with TDE


Refractive Index: 1.42 [1]

Clearing Time: Slow - days to weeks   [1]

Type: Aqueous

IHC: Compatible with immunostaining [1]

FP: Compatible with fluorescent protein   [1]

Tissue Morphology: No tissue swelling or shrinking   [1]

Carcinogenicity: No

Difficulty: Easy-to-use



TDE refers to 2,2'-thiodiethanol which is a solvent that has been used commonly to replace FocusClear used in refractive index matching applications like optical clearing with CLARITY. TDE is much more inexpensive than FocusClear and is also compatible with immunostaining and fluorescent protein. However, TDE clears much more slowly than FocusClear as it takes days to weeks instead of a few days to clear samples [1]. One the problems with TDE is that it can be challenging to obtain depending upon what country you are in as TDE is a Chemical Weapons Convention Schedule 2 Chemical as it can be used to produce mustard gas.