Visikol HISTO Buffer Recipes


Recipes for 100 mL - prepare in a volumetric flask

Buffer Name Composition Recipe for 100 mL Storage
PBS-TX100 PBS (1X) 200 mg Triton™ X-100 X-100 RT
Triton™ X-100 (0.2% wt.) Dilute to 100 mL with 1X PBS
PBST PBS(1X) 200 mg Tween® 20 RT
Tween® 20 (0.2% wt.) Dilute to 100 mL with 1X PBS
Penetration Buffer PBS 2.25 g Glycine RT
0.2% Triton™ X-100 Add 20 mL 1X PBS-TX100
0.3 M Glycine 20 mL DMSO
20% DMSO Dilute to 100mL with ~60 mL PBS-TX100
10X washing buffer (10X PTwH) 10X PBS 10 mg Heparin 4° C, 6 months.
2% Tween® 20 Add 20 mL 10X PBS Add 0.05% sodium azide for long term storageǂ
100 ug/mL Heparin 2 g Tween 20
Dilute to 100 mL with 10X PBS
Blocking Buffer PBS 6 mL Donkey Serum 4° C
0.2% Triton™ X-100 Add 10 mL PBS-TX100 Add 0.05% sodium azide for long term storageǂ
6% Donkey Serum Add 10 mL DMSO
10% DMSO Dilute to 100 mL with PBS-TX100
Antibody Buffer PBS 3 mL Donkey Serum 4° C
0.2% Tween 20 Add 20 mL 1X Washing Buffer Add 0.05% sodium azide for long term storageǂ
Heparin Add 5 mL DMSO
3% Donkey Serum Dilute to 100 mL with 1X Washing Buffer
5% DMSO

* To make 100 mL of 1X washing buffer, dilute 10 mL 10X buffer with 90 mL of DI H2O

ǂ Sodium azide will interfere with horseradish peroxidase-based protocols, but has no effect on immunofluorescent labeling

Catalog numbers

1.     Triton™ X-100 – Fisher Scientific – Catalog # BP151

2.     Tween® 20[i] – Fisher Scientific – Catalog # BP337

3.     Glycine – Fisher Scientific – Catalog # G46

4.     DMSO – Fisher Scientific – Catalog # BP231

5.     Heparin – Alfa Aesar – Catalog # A16198

6.     Normal Donkey Serum – Jackson Immunoresearch – Catalog #: 017-000-121

i.    Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow.

ii.  Tween is a registered trademark of Croda International PLC

[i] Tween is a registered trademark of Croda International PLC