As modern medicine becomes more and more complex, there is a growing need for researchers to understand the body’s response to different disease states and the complex interplay between the disease and the immune system. One of the many tools that researchers can use to visualize cell populations and protein distribution is fluorescent immunohistochemistry which allows for many markers to be visualized simultaneously. At Visikol, we have developed a proprietary multiplex slide imaging approach which allows us to visualize over ten markers per slide by conducting sequential rounds of 3-5 marker labeling and imaging. Today, we are excited to launch a new webpage that details some of our validated multiplex panels which can be combined together. These panels were developed to interrogate the complex tumor-immune environment, with 16 immune and cancer cell biomarkers. 

Each antibody in each panel has been fully validated and checked for cross-reactivity against the other biomarkers. The first panel is the T-cell Activation Panel which includes DAPI, PanCK, CD3, Granzyme b, and Ki67. The second panel is the PD-L1 Checkpoint Inhibition Panel with the following markers: DAPI, CD68, PanCK, CD8, and PD-L1. The third panel is the Antigen Presenting Cell Panel with DAPI, CD68, MHCII, CD20, and CD11b. The fourth panel is called the Memory T-cell Panel and includes DAPI, PD-1, PanCK, CD3, and CD45RO.  

The antibodies of each panel are interchangeable with others, allowing researchers to create their own panels. The proprietary Visikol antibody stripping approach allows our team to confirm that there is no left-over fluorescence on the tissues in between labeling rounds which produces repeatable, high quality image sets. In tandem to the interchangeability, Visikol can create custom panels for any research question. Our team can work with any antibody that has been validated for use in immunohistochemistry and often develops custom panels for clients.  

If you want to learn more, visit our Multiplex Technology Page, our Overview Page, or our recent blog post about the Clinical Applications of Multiplex Imaging. 

And of course, please check out our new webpage detailing each of the panels in more detail and stay tuned for more panels coming in the near future!