Over the last few years, several technological approaches have been developed to provide researchers with the ability to survey the immune system landscape on slides. However, many of these technologies require expensive imaging or tissue processing equipment and the use of proprietary labeling techniques. Other techniques such as bleaching do not completely remove fluorescence which makes achieving multiple round of labeling challenging. The significant upfront costs and reliance on proprietary antibody technologies have limited the widespread use. Since most techniques rely on proprietary antibodies, the necessity to re-validate antibody panels increases the barrier to adoption for using these approaches.
Due to the need for a robust multiplexing technique which is compatible with any antibody, Visikol has developed a unique approach to highly-multiplexed immunohistochemistry. The approach is compatible with any antibody that has been validated for use in immunohistochemistry. To accomplish multiple rounds of labeling, our technology leverages our EasyPlex™ Antibody Stripping Reagent which gently denatures primary and secondary antibodies without damaging the tissue or disrupting antigenicity. After multiple rounds of labeling and imaging are conducted, the data sets are aligned using computational co-registration to achieve a single dataset with 10+ channels. From this dataset, systematic quantification of cells of interest (e.g. immune cells) and complex spatial analysis to reveal interactions and relationships between cell populations and structures within the tissue.