Over the last few years, several technological approaches have been developed to provide researchers with the ability to survey the immune system landscape on tissue sections. However, many of these technologies require expensive imaging or tissue processing equipment, and the use of proprietary labeling techniques. Other techniques, such as bleaching which has been shown to not completely remove fluorescence. Making achieving successful multiple rounds of labeling challenging. The significant upfront costs and reliance on proprietary antibody technologies have limited the widespread use. Since most techniques rely on proprietary antibodies, the necessity to re-validate antibody panels increases the barrier to adoption for using these approaches.
Due to the need for a robust multiplexing technique which is compatible with any antibody, Visikol has developed a unique approach to highly multiplexed immunohistochemistry.