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The Advantages of Highly Multiplexed IHC Services

Carol Tomaszewski — Tue, 18 Jun 2024 13:42:05 +0000

Tissue profiling is a critical aspect of drug discovery and development, as it involves analyzing the molecular composition of tissues to identify new drug targets, biomarkers, and therapeutic interventions. Traditional methods of tissue profiling, such as single-marker immunohistochemistry, have limitations in terms of the number of markers that can be analyzed simultaneously, making it challenging to obtain a comprehensive understanding of complex biological systems. However, Visikol’s highly-multiplexed immunohistochemistry services offer a game-changing solution to this problem, providing scientists with a powerful tool to analyze multiple molecular markers in a single tissue sample.

Unprecedented Detail of Samples

Visikol’s highly-multiplexed immunohistochemistry services allow for the profiling of tissues with 10+ markers on a single slide, providing unprecedented detail about samples. Using their proprietary EasyPlex antibody stripping reagent and co-registration software, multiple rounds of sequential labeling can be conducted from the same slide, thereby reducing costs and increasing efficiency. The service uses fluorescent slide scanning or confocal microscopy to image labeled samples in 5 channels for each round at 10x, 20x, or 40x. The imaging data acquired from multiple rounds of labeling is co-registered using custom-built image processing algorithms to create a highly-multiplexed fluorescence image stack. Quantitative analysis of image data allows for the measurement of cell populations at unprecedented detail within a single sample.

Advanced Imaging and Analysis

Visikol’s highly-multiplexed immunohistochemistry services rely on advanced imaging and analysis techniques, including digital slide imaging and AI-enhanced image analysis, to capture a large number of valuable endpoints from a single specimen. Digital slide imaging allows for high-resolution scanning of whole slide images, providing a permanent digital record of the sample. This eliminates the need for repeated staining and enables remote access to the images by researchers and clinicians worldwide. AI-enhanced image analysis utilizes machine learning algorithms to detect and quantify cells and structures of interest, reducing the time and effort required for manual analysis. This approach enables the identification of subtle changes in tissue composition and architecture, which can be used to develop novel biomarkers and therapeutic targets.

Customized Research

Visikol’s highly-multiplexed immunohistochemistry services are highly versatile and can be customized to suit the specific research needs of scientists working in pharma or medical research. Visikol offers validated panels of antibodies designed for immunological profiling, or clients can work with their team to design a custom panel to suit their needs. This allows for flexibility in terms of the type of markers analyzed, the number of rounds of labeling, and the imaging resolution used. The ability to customize the services ensures that scientists obtain the data they need to make informed decisions about drug development, clinical trials, and patient care. If you’re interested in learning more, please reach out to a member of our team today!

The post The Advantages of Highly Multiplexed IHC Services first appeared on Visikol.

Immunological Profiling Made Easy: The Advantages of Validated Antibody Panels

Carol Tomaszewski — Wed, 16 Aug 2023 12:19:24 +0000

As medical research becomes increasingly complex, the need for a more comprehensive understanding of the biological processes underlying disease has become more important than ever. This has led to the development of new technologies and techniques that allow for more detailed and accurate analysis of tissue samples. One such technology is multiplex immunohistochemistry, which has revolutionized how researchers analyze tissue samples.

Multiplex Immunohistochemistry

Multiplex immunohistochemistry is a technique that allows researchers to simultaneously visualize and quantify multiple proteins within a single tissue sample. This is achieved by using multiple fluorescently labeled antibodies that bind to specific proteins of interest. Each antibody is labeled with a different fluorescent dye, which allows for the visualization of multiple proteins within the same tissue section.

One of the challenges of multiplex immunohistochemistry is the need to validate each antibody to ensure that it binds specifically to its target protein. This can be a time-consuming and expensive process, but it is critical to ensure the accuracy and reproducibility of the results. To address this challenge, Visikol offers validated antibody panels as part of their highly-multiplexed immunohistochemistry services.

Validated Antibody Panels

Visikol’s validated antibody panels are designed to provide actionable insights in an easy-to-read report, allowing clients to make decisions and push forward their research efforts. Each panel is designed to target a specific set of proteins that are relevant to a particular disease or biological process. By using a validated panel, researchers can be confident that each antibody in the panel has been thoroughly tested and validated, which saves time and resources.

These validated antibody panels are also highly versatile and can be customized to suit the specific research needs of scientists working in pharma or medical research. This flexibility allows researchers to design a panel that is tailored to their specific research question, which can lead to more accurate and relevant results.

Advanced Imaging Services

In addition to the validated antibody panels, Visikol also offers a range of advanced imaging services, including digital slide imaging. Digital slide imaging allows for the high-resolution imaging of tissue samples, which can be analyzed using custom-built image processing algorithms. This allows for the creation of a highly-multiplexed fluorescence image stack, which can be analyzed using quantitative analysis to measure cell populations at unprecedented detail within a single sample.

Multiplex immunohistochemistry is a powerful technique that allows for the simultaneous visualization and quantification of multiple proteins within a single tissue sample. Visikol’s validated antibody panels provide a streamlined and cost-effective way to perform this technique while ensuring the accuracy and reproducibility of the results. The flexibility of the panels also allows for customization to suit the specific research needs of scientists working in pharma or medical research. With Visikol’s highly-multiplexed immunohistochemistry services and advanced imaging capabilities, researchers can gain a more comprehensive understanding of the biological processes underlying disease, which can lead to the development of new and more effective treatments.

To learn more about Visikol’s Highly-Multiplexed Immunohistochemistry Services, please reach out to a member of our team today.

The post Immunological Profiling Made Easy: The Advantages of Validated Antibody Panels first appeared on Visikol.

The Advantages of Multiplex IHC in Drug Discovery

Carol Tomaszewski — Thu, 01 Jun 2023 18:18:13 +0000

Immunohistochemistry (IHC) is a widely used technique for visualizing the presence and localization of specific cellular components in tissue samples. In drug discovery and development, IHC is used to profile tissues and determine biomarker expression, which can provide valuable insights into the mechanisms of drug action, toxicity, and resistance. However, traditional IHC has limitations when it comes to multiplexing – the ability to label multiple markers in the same sample. This is where Visikol’s Highly-Multiplexed Immunohistochemistry Services come in.

Visikol’s technology allows for the labeling of 10+ markers on a single slide and co-registration of multiple rounds of sequential labeling from the same slide. This means that pharmaceutical scientists can obtain unprecedented detail about samples, providing a more comprehensive understanding of tissue biology and drug activity. In this blog post, we will explore how Visikol’s Highly-Multiplexed Immunohistochemistry Services can revolutionize drug discovery and development.

The Limitations of Traditional IHC

Traditional IHC methods have been used for decades to visualize the presence and localization of proteins in tissue samples. These methods use a single primary antibody specific to the protein of interest, which is then detected using a secondary antibody conjugated to an enzyme or a fluorescent label. While this technique is useful for visualizing single proteins, it has limitations when it comes to multiplexing.

Multiplexing with traditional IHC requires the use of different primary antibodies, each with a unique detection system. This can lead to issues with cross-reactivity and background noise, making it difficult to obtain accurate and reproducible results. Additionally, traditional IHC methods are limited in the number of markers that can be labeled at once, typically no more than 3 or 4. This limitation makes it difficult to obtain a comprehensive understanding of tissue biology and drug activity.

The Advantages of Multiplex Immunohistochemistry

Multiplex immunohistochemistry (mIHC) overcomes the limitations of traditional IHC by allowing the labeling of multiple markers in the same sample. This technique is particularly useful for tissue profiling, where the expression of multiple biomarkers can provide valuable insights into tissue biology and drug activity.

Visikol’s Highly-Multiplexed Immunohistochemistry Services take mIHC to the next level, allowing for the labeling of 10+ markers on a single slide. This is achieved through the use of Visikol’s proprietary EasyPlex™ antibody stripping reagent and co-registration software, which enable multiple rounds of sequential labeling from the same slide. This means that multiple markers can be labeled and imaged in the same sample, providing unprecedented detail about tissue biology and drug activity.

How Multiplex Immunohistochemistry Can Revolutionize Drug Discovery

The ability to label multiple markers in the same sample provides pharmaceutical scientists with unprecedented insights into the mechanisms of drug action, toxicity, and resistance. By profiling tissues with Visikol’s Highly-Multiplexed Immunohistochemistry Services, scientists can obtain a more comprehensive understanding of how drugs interact with tissues and cells, allowing for the development of more effective drugs with fewer side effects.

Visikol’s technology is particularly useful for customized research, where clients can work with Visikol’s team to design a custom panel of markers to suit their research needs. This means that pharmaceutical scientists can obtain insights into specific pathways and mechanisms of interest, allowing for more targeted drug development.

Multiplex immunohistochemistry is a powerful tool for tissue profiling in drug discovery and development. Visikol’s Highly-Multiplexed Immunohistochemistry Services provide unprecedented detail about tissue biology and drug activity, allowing for more effective drug development with fewer side effects. By offering customized research services, Visikol is revolutionizing the field of tissue profiling and drug discovery.

If you’re interested in learning more about Visikol’s Highly-Multiplexed Immunohistochemistry Services, please reach out today!

The post The Advantages of Multiplex IHC in Drug Discovery first appeared on Visikol.