Modern medicine requires detailed understanding not only of the disease, but of the body’s response to it. A deeper understanding of cellular interactions within the context of cancer and inflammatory diseases is critical to developing better treatments and therapeutic outcomes. With advances in fluorescent probes and imaging technologies, fluorescent immunohistochemistry is a powerful tool to probe cell populations and protein distribution within tissues, but are fundamentally limited by the number of markers that can be assessed simultaneously.
Traditionally, researchers have been limited to the number of markers they can use simultaneously when characterizing slides due to the inherent limitations of fluorescent or chromogenic labeling. At Visikol, we provide our Clients with the ability to move beyond traditional slide labeling and imaging such that they can achieve 10+ labeling from a single slide. We accomplish this using our proprietary EasyPlex™ antibody stripping reagent and co-registration software that allows for antibodies to be removed from tissues such that multiple rounds of sequential labeling can be conducted from the same slide.