Quantification of the total number of cells within histological sections is a common measure for a range of applications. Manual cell counting is laborious and prone to operator error, and as such, quantification via computerized image analysis is a preferred method for establishing cell counts. Quantification of the number of cells within the tumor boundary, the number of hormone receptor positive cells, or quantification of the number of T cells that have invaded into a tumor or frequently used assessments conducted on histological sections.
Immunohistochemistry is frequently conducted to determine the extent of and changes to populations of cells, to examine for the presence of receptors and markers which are indicated in diseases, and to evaluate the effect of drug treatments on animal and human tissues. Traditionally, analysis of slides prepared with IHC techniques involved subjective analysis by a pathologist, often including a score indicating the extent of the presence or absence of a given marker. With modern computational techniques and image processing, analysis of IHC sections can be accomplished quantitatively on large numbers of tissue sections in a fraction of the time.
Visikol offers a suite of services for the quantification and analysis of H&E and IHC sections, to examine the extent and distribution of cells relevant to disease pathology. Quantification of the breakdown of subpopulations of cells, determination of the coincidence and spread of distances between cell types of interest, and examination of the changes to protein expression of specific cell types, to name a few examples, are accomplished through the use of automated image processing.