The 2nd Annual Cell Biology Virtual Event will focus on the recent discoveries in biological research, advancements in techniques, and tool developments in cell research. The overall focus of the event is to discuss the following topics:
- Methodologies and their effect on results
- Organ on a chip
- Advanced tech, emerging trends
- Microscopy
- Translation approaches
- Stem cell
- Cell signaling
- Cilia / Ciliopathy
- Cellular polarity
This field spans through most scientific disciplines, studying cell imaging, stem cells, cell physiology and structure, cell signaling, cell culture, and translational biology, and encompassing other scientific fields such as cancer biology, neuroscience, genetics, bioengineering and so much more. The free, two-day conference will bring together professors, post-docs, biologists and biomedical professionals from all over the world. Attend interactive sessions, first-class exhibitions and virtual poster presentations.
Dr. Michael Johnson from Visikol will discuss how best to characterize 3D cell culture models (e.g. organoids, spheroids, microtissues) and considerations for using 3D cell culture models in assays:
Abstract
With the advent of cost-effective culturing approaches, 3D cell culture models (3D-CCMs) have been rapidly adopted for drug discovery since they provide a more physiologically relevant micro-environment; showing improved predictive utility for assessing drug efficacy and/or toxicity when compared to traditional 2D monolayer models. High-content analysis/screening (HCA/HCS) also plays a major role in drug screening, but one of the unique challenges of evaluating 3D-CCMs (i.e. spheroids, organoids, micro-tissues, organs-on-a-chip) is their opacity and thickness that limit optical imaging to only the outermost layers (~20=30 microns). Since the outermost cells are exposed to different physiological test conditions (oxygen, nutrients, media exchange, drug dosing), current image-based approaches induce a bias in the results since the outer layer of cells are found in a significantly different micro-environment than the interior. This shortcoming is particularly problematic when ascertaining the relative effectiveness of current therapeutic agents (e.g. immunoglobulin-based therapeutics, anti-proliferatives) since their effects are likely to be localized or concentrated to the surface while their efficacy within the interior are obfuscated and not fully resolved. Visikol™ has shown that through the addition of high content confocal microscopy with the Thermo Scientific™ CellInsight™ CX7 LZR High-Content Analysis Platform and the Visikol® HISTO-M™ reagent that the entire population of cells within 3D-CCMs can be characterized.
To register for this event and to check out the complete schedule click below:
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