Figure 1. Maximum Z-projection of Z-stack obtained from High Content Imaging of tumor spheroids in the presence of activated and naive immune cells. Data was quantified by automated image analysis to count total number of cells, and number of infiltrating immune cells.
This video depicts a representation of how immune cell infiltration is measured in 3D. Each immune cell is mapped to its position within the 3D cell model and the distance to the boundary is measured. Dark lines indicate cells that are close to the surface, orange/yellow lines depict cells that are far into the interior.