Validated Kits

Validated Kits2019-07-23T16:04:24-05:00

We recognize that one of the most challenging parts of adopting tissue clearing into a research workflow is the optimization of immunolabeling for a specific type of tissue. While many researchers want to label, clear and image a whole tissue such as a mouse brain, there is seldom ever a need to do this and instead the 3D reconstruction of a whole tissue from several 1 mm sections will dramatically reduce processing time and cost while improving results. Below, we have partnered with Abcam to generate several application specific protocols inclusive of labeling guidance.

Protocols for 1mm mouse brain immunolabeling and tissue clearing

Synapsin I Protocol

Product Name: Rabbit Polyclonal to Synapsin I (ab64581)

Description: Tissue labeling kit for Synapsin I

Host Species: Rabbit IgG

Size: 1 mm thick coronal section of mouse brain

Iba1 Protocol

Product Name: Rabbit monoclonal [EPR16589] to Iba1 (ab178847)

Description: Tissue labeling kit for Iba1

Host Species: Rabbit IgG

Size: 1 mm thick coronal section of mouse brain

GFAP Protocol

Product Name: Rabbit Polyclonal to GFAP (ab7260)

Description: Tissue labeling kit for GFAP

Host Species: Rabbit IgG

Size: 1 mm thick coronal section of mouse brain

NeuroD1 Protocol

Product Name: Rabbit Monoclonal to NeuroD1 (ab213725)

Description: Tissue labeling kit for NeuroD1

Host Species: Rabbit IgG

Size: 1 mm thick coronal section of mouse brain

Parvalbumin Protocol

Product Name: Rabbit Polyclonal to Parvalbumin (ab94362)

Description: Tissue labeling kit for Parvalbumin

Host Species: Rabbit IgG

Size: 1 mm thick coronal section of mouse brain

NeuN Protocol

Product Name: Rabbit Monoclonal to NeuN (ab177487)

Description: Tissue labeling kit for NeuN

Host Species: Rabbit IgG

Size: 1 mm thick coronal section of mouse brain

S100 B Protocol

Product Name: Rabbit Monoclonal to S100 beta (ab52642)

Description: Tissue labeling kit for S100 beta

Host Species: Rabbit IgG

Size: 1 mm thick coronal section of mouse brain

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