What is Visikol HISTO?
Visikol HISTO is a novel tissue clearing approach for the 3D visualization of tissues that is non-destructive, rapid, and easy-to-use. Unlike many other clearing techniques, Visikol HISTO does not require the removal of cell-membrane lipids, and as such, preserves innate cellular structures. This allows the clearing effect to be reversed, and the tissue to be used in downstream assays. Visikol HISTO does not require embedding into polyacrylamide gel as in CLARITY and related methods, does not require perfusion of the animal tissue, or the use of special fixatives. In addition to being compatible with fluorescent protein, we have developed an accompanying immunolabeling procedure that is carried out prior to clearing with Visikol HISTO.
How does Visikol HISTO work?
Visikol HISTO renders tissues transparent because the chemical formula allows the reagent to penetrate cell-membranes, permeating the cytosolic and intercellular space. Through this process, the refractive index of the cytosolic and intercellular space is increased to match the refractive index of cell-membranes and proteins (at an RI of 1.47 to 1.51), and when equilibrated, tissues become transparent. Because Visikol HISTO has been carefully optimized to penetrate cellular structure without damaging cells, tissues cleared with Visikol HISTO can be restored to their initial state, allowing follow up assays and histology on three-dimensionally imaged tissues.