Immunohistochemistry (IHC), immunofluorescence (IF) and histological stains are a powerful tool for the examination of the distribution of relevant biomarkers and structural changes in tissue samples. Immunohistochemistry is frequently conducted to determine the extent of and changes to populations of cells, to examine for the presence of receptors and markers which are indicated in diseases, and to evaluate the effect of drug treatments on animal and human tissues. Traditionally, analysis of slides prepared with IHC techniques involved subjective analysis by a pathologist, often including a score indicating the extent of the presence or absence of a given marker. With modern computational techniques and image processing, analysis of IHC sections can be accomplished quantitatively on large numbers of tissue sections in a fraction of the time.
Visikol offers a suite of services for the quantification and analysis of IHC sections, to examine the extent and distribution of biomarkers important in disease pathology. Evaluation of the colocalization of various biomarkers allows for quantification of the breakdown of subpopulations of cells, determination of the coincidence between biomarkers of interest, and examination of the changes to protein expression of specific cell types.