Below is a non-comprehensive list of antibodies that we have validated for use with the Visikol® HISTO™ reagents. We also routinely use these antibodies with traditional slide based immunolabeling for multiplex assays. The specific depth of penetration for each antibody will be dependent upon tissue type, dilution as well as incubation time. We always suggest optimizing your antibody in small sections and working your way up to your desired thickness as you optimize dilution and incubation time.
| Antigen | Host Species | Supplier | Cat # |
|---|---|---|---|
| Actin | Mouse | Invitrogen | MA511869 |
| Adenylate Cyclase III | Rabbit | EnCor Bio | RPCA-ACIII |
| Albumin | Rabbit | Abcam | ab207327 |
| Albumin | Chicken | Abcam | ab106582 |
| Alpha sarcomeric actinin | Mouse | Thermo | MA1-22863 |
| Anti-Aiolos (clone D1C1E) | Rabbit | Cell Signaling Technology | 15103S |
| Anti-Aiolos (clone D1C1E) | Rabbit | Cell Signaling Technology | 10896S |
| Anti-CD28 (clone 28.2) | Mouse | Invitrogen | 16-0289-81 |
| Anti-CD3 (clone OKT) | Mouse | Invitrogen | 14-0037-82 |
| Anti-F-actin (clone NH3) monoclonal antibody | Thermo Fisher | MA180163 | |
| Atrial natriuretic peptide (ANP) | Rabbit | Novus Biologics | NBP2-14837 |
| Beta catenin | Rabbit (monoclonal) | AbCam | ab32572 |
| Beta integrin | Rabbit (monoclonal) | AbCam | ab52971 |
| Calbindin | Chicken | EnCor Bio | CPCA-Calb |
| Calretnin | Chicken | EnCor Bio | CPCA-Calretinin |
| Caspase-3 | Mouse | Thermo | MA5-11516 |
| CD11b | Goat | Abcam | ab62817 |
| CD3 | Rabbit | Novus | NB600-1441 |
| CD3 | Mouse | BD Biosciences | 550367 |
| CD31 | Rabbit | Abacam | ab28364 |
| CD68 | Mouse | Thermo | 14-0689-80 |
| CD68 | Mouse | Invitrogen | 14068980 |
| CD68 | Rabbit | Abcam | ab53444 |
| CD68 | Rabbit | Abcam | ab125212 |
| cFOS | Rabbit | EnCor Bio | RPCA-c-Fos-AP |
| CK-14 | Mouse | Invitrogen | MA511599 |
| Collagen I | Sheep | R&D Systems | AF6220 |
| Collagen I | Mouse | Invitrogen | MA1-26771 |
| Collagen I | Rabbit | Boster Bio | PB9939 |
| Collagen III | Mouse | Thermo | CSI-007-01-02 |
| Collagen III | Rabbit | Abcam | ab7778 |
| Collagen IV | Mouse | Thermo | 14-9871-82 |
| Collagen IV | Goat | Invitrogen | PA136001 |
| Collagen IV | Rabbit | Invitrogen | PA126148 |
| Connexin 43 | Rabbit | Millipore | AB1728 |
| Cytochrome P450 3A4 | Rabbit | Abcam | ab124921 |
| E-cadherin | Rat | Thermo | 16-3249-82 |
| E-Cadherin | Rabbit (monoclonal) | AbCam | ab40772 |
| E-Cadherin | Rat | Invitrogen | 53324982 |
| eGFR | Rabbit | Invitrogen | PA1-1110 |
| eMHC (embryonic myosin) | Mouse | DSHB | F1.652-s |
| ErbB2 (HER2) | Rabbit | Invitrogen | PA514635 |
| Estrogen Receptor | Rabbit | Thermo | PA5-16440 |
| Estrogen Receptor | Mouse | Thermo | MA1-80216 |
| F4/80, clone BM8 | Rat | Invitrogen | 14-4801(-85) |
| Fox3/NeuN | Mouse (monoclonal) | EnCor Bio | MCA-1B7 |
| Fox3/NeuN | Rabbit | EnCor Bio | RPCA-Fox3 |
| GFAP | Chicken | Encore | CPCA-GFAP |
| GFAP | Rabbit | Encore | RPCA-GFAP |
| GFAP | Chicken | EnCor Bio | CPCA-GFAP |
| GFAP | Mouse (monoclonal) | EnCor Bio | MCA-5C10 |
| Ki-67 | Rabbit | AbCam | ab15580 |
| Ki-67 | Rabbit (monoclonal) | AbCam | av16667 |
| Ki-67 | Rabbit | Thermo Scientific Lab Vision | RB1510 |
| MDRI | Rabbit | Abcam | ab170904 |
| Methy CPG-Binding Protein 2 | Rabbit | EnCor Bio | RPCA-MeCP2 |
| MRPII | Mouse | Thermo | MA1-26535 |
| Myelin Basic Protein | Chicken | EnCor Bio | CPCA-MBP |
| Myelin Basic Protein | Mouse (monoclonal) | EnCor Bio | MCA-7G7 |
| N-cadherin | Thermo | PA5-17526 | |
| NeuN | Rabbit | AbCam | ab177487 |
| NeuN-Alexafluor 488 Conjugate | Rabbit | Millipore | ABN-90 |
| NeuroD1 | Rabbit | Abacam | ab213725 |
| Neurofilament-Heavy Chain | Mouse (monoclonal) | EnCor Bio | MCA-AH1 |
| Neurofilament-Heavy Chain | Chicken | EnCor Bio | CPCA-NFH |
| Neurofilament-Medium Chain | Rabbit | EnCor Bio | RCPA-NF-M |
| Pan Collage | Rabbit | Thermo | PA136058 |
| Pan Cytokeratin | Mouse | Thermo | MA5-13156 |
| Pan-Cytokeratin Alexa-488 | Mouse | Invitrogen | 53900380 |
| Parvalbumin | Mouse (monoclonal) | Swant | PV-235 |
| PDGF-Beta | Rabbit | Invitrogen | PA511723 |
| PDGFR-B1 | Rabbit | Thermo | MA5-15143 |
| PDX-1 | Guinnea Pig | Abcam | ab47308 |
| PDX-1 | Guinea Pig | Abcam | 47308 |
| PHH3 | Rabbit | Invitrogen | PA517869 |
| Pro-Caspase 3 | Mouse | Invitrogen | MA141163 |
| Progesterone Receptor | Chicken | Sigma | GW22679 |
| RECA-1 | Mouse | BioRad | MCA970GA |
| Troponin T | Mouse | Thermo | MS-295-P1 |
| VGluT1 | Mouse | Abcam | ab134283 |
| Vimentin | Chicken | EnCor Bio | CPCA-Vime |
| Vimentin | Mouse (monoclonal) | EnCor Bio | MCA-2D1 |
| Vimentin | Rabbit | Invitrogen | PA527231 |
| Vimentin | Chicken | Thermo | PA1-10003 |
| α-SMA | Rabbit | Abcam | ab32575 |