HUREL® Dog™2021-10-14T12:39:44-05:00

HUREL® Dog™

Co-culture comprised of cryopreserved primary dog hepatocytes cultured with cells of non-parenchymal, stromal type.

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Example Metabolic Activity (nmoles/hr/106 cells)

SubstrateEnzymeConcentration (µM)Day 1Day 4Day 8
7-EthoxycoumarinPhase I250.070.130.22
7-HydroxycoumarinPhase II1009.1819.589.24
7-HydroxycoumarinPhase III1002.56.94.11

Culture Condition and Morphology

Cryopreserved primary dog hepatocytes were thawed and plated with HUREL PlatinumHeps™ Media supplemented with 10% serum and subsequently changed 24 hours post-seeding to HUREL PlatinumHeps™ Basal Media. CYP substrate concentrations are given in the table above along with metabolite formation recorded as nmoles/hr/106 cells. All incubations were carried out in triplicate on days D1, D4, and D8 upon cell delivery and incubated for 60 minutes. Reactions took place in a humidified incubator at 37°C, in 5% CO2. Col­lected supernatants were stored at -20°C until further LC/MS/MS analysis.

Day 1 – Morphology

day-1-dog

Phase contrast image in a 96-well at a 10x magnification

Day 7 – Morphology

Phase contrast image in a 96-well at a 10x magnification

Day 7 – Bile Canaliculi

day-7-dog-bile

Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (CDFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 96-well at 10x magnification

Example Culture Origin

Dog Donor Demographics
  • Strain: Beagle
  • Number of Donors: 1
  • Age: 5 months
  • gender: Male
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