HUREL® Mouse™2021-10-20T14:31:08-05:00

HUREL® Mouse™

Co-culture comprised of cryopreserved primary mouse hepatocytes cultured with cells of non-parenchymal, stromal type.

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Example Metabolic Activity (nmoles/hr/106 cells)

Substrate

Enzyme

Concentration (µM)

Day 1

Day 4

Day 8

7-EthoxycoumarinPhase I1000.0340.0170.016
7-HydroxycoumarinPhase II10051.546.50.21
7-HydroxycoumarinPhase III1001.761.020.08

Culture Condition and Morphology

Cryopreserved primary mouse hepatocytes were thawed and plated with HUREL PlatinumHeps™ Media supplemented with 10% serum and subsequently changed 24 hours post-seeding to HUREL PlatinumHeps™ Basal Media. CYP substrate concentrations are given in the table above along with metabolite formation recorded as nmoles/hr/106 cells. All incubations were carried out in triplicate on days D1, D4, and D8 upon cell delivery and incubated for 60 minutes. Reactions took place in a humidified incubator at 37°C, in 5% CO2. Col­lected supernatants were stored at -20°C until further LC/MS/MS analysis

Day 1 – Morphology

Phase contrast image in a 24-well at a 10x magnification.

Day 7 – Morphology

HUREL Mouse 2

Phase contrast image in a 24-well at a 10x magnification.

Day 7 – Bile Canaliculi

Mouse 3

Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (C-DCFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 96-well at 10x magnification with filters EX/EM 492-495/512-527 nm.

Example Culture Origin

Animal Donor Demographics
  • Strain: CD-1
  • Number of Donors: 5
  • Age(yrs)
  • Sex: Male
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