HUREL® Rat WH™

HUREL® Rat WH™2023-06-14T11:09:15-05:00

HUREL® Rat WH™

Co-culture comprised of cryopreserved primary rat hepatocytes cultured with cells of non-parenchymal, stromal type.

Example Metabolic Activity (nmoles/hr/106 cells)

Substrate

Enzyme

Concentration (µM)

Day 1

Day 4

Day 8

7-EthoxycoumarinPhase I(ECOD)C1000.050.050.05
Phase II(UGT)0.050.050.05
Phase II(SULT)0.050.050.05
7-HydroxycoumarinPhase II(UGT)1000.050.050.05
Phase II(SULT)0.050.050.05

Culture Conditions and Morphology

Cryopreserved rat hepatocytes were thawed and plated with Hurel PlatinumHeps™ Media supplemented with 10% serum and subsequently changed 24 hours post-seeding to Hurel PlatinumHeps™ maintenance Media. CYP substrate concentrations are given in the table above along with metabolite formation recorded as nmoles/hr/million cells. All incubations were carried out in triplicate on days 1, 4, and 8 (customer day) upon cell delivery and incubated for 60 minutes. Reactions took place in a humidified incubator at 37°C, in 5% CO2. Collected supernatants were stored at – 20°C until further LC/MS/MS analysis.

Day 1 – Morphology

Morphology-Human-1-2

Phase contrast image in a 24-well at a 10X magnification

Day 7 – Morphology

Morphology-Human-2-2

Phase contrast image in a 24-well at a 10X magnification.

Day 7 – Bile Canaliculi

Morphology-Human-3-2

Bile canaliculi assayed via 5-(and-6)-carboxy-2′, 7′ – dichlorofluorescein diacetate (C-DCFDA) stain at a concentration of 5 microns and imaged in the GFP channel in a 96-well at 10X magnifcation with filters EX/EM 492-495/512-527 nm.

Example Culture Origin

Animal Donor Demographics

  • Strain: Wistar Han
  • Number of Donors:  15
  • Age: 2 months
  • Gender: Male