We recognize that one of the most challenging parts of adopting tissue clearing into a research workflow is the optimization of immunolabeling for a specific type of tissue. While many researchers want to label, clear and image a whole tissue such as a mouse brain, there is seldom ever a need to do this and instead the 3D reconstruction of a whole tissue from several 1 mm sections will dramatically reduce processing time and cost while improving results. Below, we have partnered with Abcam to generate several application specific protocols inclusive of labeling guidance.
Protocols for 1mm mouse brain immunolabeling and tissue clearing
Synapsin I Protocol
Product Name: Rabbit Polyclonal to Synapsin I (ab64581)
Description: Tissue labeling kit for Synapsin I
Host Species: Rabbit IgG
Size: 1 mm thick coronal section of mouse brain
Iba1 Protocol
Product Name: Rabbit monoclonal [EPR16589] to Iba1 (ab178847)
Description: Tissue labeling kit for Iba1
Host Species: Rabbit IgG
Size: 1 mm thick coronal section of mouse brain
GFAP Protocol
Product Name: Rabbit Polyclonal to GFAP (ab7260)
Description: Tissue labeling kit for GFAP
Host Species: Rabbit IgG
Size: 1 mm thick coronal section of mouse brain
NeuroD1 Protocol
Product Name: Rabbit Monoclonal to NeuroD1 (ab213725)
Description: Tissue labeling kit for NeuroD1
Host Species: Rabbit IgG
Size: 1 mm thick coronal section of mouse brain
Parvalbumin Protocol
Product Name: Rabbit Polyclonal to Parvalbumin (ab94362)
Description: Tissue labeling kit for Parvalbumin
Host Species: Rabbit IgG
Size: 1 mm thick coronal section of mouse brain
NeuN Protocol
Product Name: Rabbit Monoclonal to NeuN (ab177487)
Description: Tissue labeling kit for NeuN
Host Species: Rabbit IgG
Size: 1 mm thick coronal section of mouse brain
S100 B Protocol
Product Name: Rabbit Monoclonal to S100 beta (ab52642)
Description: Tissue labeling kit for S100 beta
Host Species: Rabbit IgG
Size: 1 mm thick coronal section of mouse brain