In working with hundreds of research groups from around the world, we have recognized that many research groups have tried to adopt tissue clearing and 3D tissue imaging into their research unsuccessfully. The reason for this is that there are dozens of different types of tissue clearing techniques and every protocol needs to be optimized for the specific type of tissue, fixation technique, antibody, tissue thickness and microscope that is being used by a researcher. This is the reason we ultimately developed our Visikol® HISTO™ tissue clearing technique so that researchers could have an easy-to-use technique that could be applied to a wide range of applications. Below we provide guidance on how best to use the Visikol HISTO tissue clearing technique for various research questions and also provide general guidance on many topics such as 3D tissue imaging through the use of light sheet and confocal microscopy.
Visikol HISTO Getting Started
Prior to using the Visikol HISTO tissue clearing technique for your research project, check out this tutorial on how best to get started to save both time and money.
Visikol HISTO Immunolabeling
Immunolabeling when paired with tissue clearing allows for unprecedented 3D tissue imaging. However, it can be challenging to master and here we provide some guidance for you.
Light Sheet Microscopy
Light sheet microscopy is ideal for imaging large tissue volumes in 3D through the use of tissue clearing and fluorescent labeling. Read about how best to use light sheet microscopy and the advantages and disadvantages of different systems in the marketplace.
Confocal Microscopy
Confocal microscopy is the most common 3D tissue imaging modality available to researchers and is a highly powerful tool. Here we describe how best to use confocal microscopy for 3D tissue imaging and some of the advantages and disadvantages of different types of systems.
Liver 3D Cell Culture Models
Liver 3D cell culture models allow for the recapitulation of in vivo biology using inexpensive and high-throughput in vitro systems.
3D Cell Culture Imaging
Dr. Michael Johnson presenting at the NIH on considerations for adopting and characterizing 3D cell culture models. Copyright – Thermo Fisher Scientific
Using Convolutional Neural Networks
Alex Magsam describes how CNN’s can be used to identify tumor areas.