This model combines two of the most well validated in vitro model systems in the entire space together to form a unique organ system on a plate which provides researchers with a tool to assess the optimal route for therapeutic administration.
Visikol is now offering this First Pass Metabolism Model to clients as part of its comprehensive contract research services to assess the metabolism of potential therapeutics.
Cell Culture Inserts
MatTek Multi-Well Plates
The First Pass Metabolism Model uses MatTek 96-well glass bottom dishes. These dishes have a gas plasma treatment and collagen coating surface modification on the bottom plate.
Human Intestine (control): MatTek 96w
Human Intestine/Liver (first pass metabolism model): HUREL micro liver
Used MatTek 96 transwell plate for the Intestine/Liver cell culture -Bottom plate: surface treatment and collagen Type I coating
Dosing volume: -Upper compartment: 100 ul dosing compounds -Lower compartment: 300 ul dosing media (HUREL)
Time points: t=24hr
Drug Clearance (5 uM)
Drug Permeability (10 uM)
Drug Metabolism: Cytochrome P450 enzymes
Significantly higher metabolite formation in the First-Pass Metabolism Model as shown by CYP3A4, CYP2D6, CYP1A2 and CYP2C9 activity as well as Phase II metabolism.
Higher clearance (lower bioavailability) of drugs in first pass metabolism model as demonstrated by Bupropion, Verapamil, Diazepam, Repaglidine, Dextromethorphan and Troglitazone.