Characterize your whole 3D model with Visikol® HISTO-M™


Widefield Imaging

NCI-H2170 spheroids approx 250 um in diameter labeled with SYTOX green nuclear stain. Left is in PBS and right is the same spheroid after clearing with Visikol HISTO-M.

NCI-H2170 spheroids approx 250 um in diameter labeled with SYTOX green nuclear stain. Left is in PBS and right is the same spheroid after clearing with Visikol HISTO-M.

Confocal Imaging

NCI-H2170 spheroids approx 250 um in diameter labeled with SYTOX green nuclear stain. Left is in PBS and right is the same spheroid after clearing with Visikol HISTO-M.

NCI-H2170 spheroids approx 250 um in diameter labeled with SYTOX green nuclear stain. Left is in PBS and right is the same spheroid after clearing with Visikol HISTO-M.


Get 3-4 fold more data from your models


While 3D cell culture models are being rapidly adopted in the in vitro drug discovery space for their improved in vivo relevancy, researchers are currently using imaging modalities for characterization originally designed for 2D monolayer models. Confocal and widefield imaging of 3D cell culture models (e.g. spheroids, organoids, microtissues) is currently limited to only characterizing the periphery of these models. This current paradigm biases results as only cells most exposed to a compound of interest are evaluated and only a small percentage of the overall population of cells is surveyed. 

 

3D cell culture in PBS

 

 

3D cell culture in Visikol HISTO-M

 

 

Number of cells characterized

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To address these limitations, Visikol HISTO-M tissue clearing was developed for high-throughput and plate compatible tissue clearing. The combination of Visikol HISTO-M with fluorescent labeling allows for a 3-4 fold increase in the number of cells chararcetrized in 3D cell culture models. 


More sensitive and less biased insights


NCI-H2170 spheroids dosed with cisplatin and evaluated for percent Ki67 (cell proliferation) positive cells.

NCI-H2170 spheroids dosed with cisplatin and evaluated for percent Ki67 (cell proliferation) positive cells.

 

The application of tissue clearing to 3D cell culture characterization has been shown to increase dose-response sensitivity up to an order of magnitude. This increased sensitivity is possible as the entire population of cells within these models is able to be characterized with Visikol HISTO-M instead of just the cells on the periphery. 

 

Build a customized protocol for your specific research question.

Application of Visikol HISTO-M clearing with InSphero 3D InSight™ microtissues and high-throughput confocal imaging.

Application of Visikol HISTO-M clearing with InSphero 3D InSight™ microtissues and high-throughput confocal imaging.