Refractive Index: 1.47 [1]
Clearing Time: Rapid – hours to days [1]
Type: Aqueous
IHC: Compatible with immunostaining [1]
FP: Compatible with fluorescent protein [1]
Tissue Morphology: No tissue swelling or shrinking [1]
Carcinogenicity: No [1]
Difficulty: Easy-to-use
With the exception of Visikol HISTO, FocusClear™ is one of the only other commercial optical clearing agents on the market. FocusClear is a proprietary formula which was originally developed for clearing fruit fly brains and is now sold by CelExplorer Labs. FocusClear is a proprietary mixture of chemicals but its active component is diatrizoic acid. FocusClear is able to quickly render tissues transparent and is compatible with both immunolabeling and fluorescent proteins. However, FocusClear is very expensive as 5 mL costs $180 ($36 per mL)
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FocusClear publications
- Optical properties of mouse brain tissue after optical clearing with FocusClear
- Optical clearing based cellular-level 3D visualization of intact lymph node cortex
- Optical histology: a method to visualize microvasculature in thick tissue sections of mouse brain
- Seeing through Musculoskeletal Tissues: Improving In Situ Imaging of Bone and the Lacunar Canalicular System through Optical Clearing
- A versatile clearing agent for multi-modal brain imaging