Dr. Jay Deng from Ecor Bio has applied the Visikol® HISTO™ methodology to visualize several EnCor Bio immunolabels in 3D. Below you can see the penetration depth for each immunolabel as well as some z-stacks obtained from tissue using confocal microscopy with our standard immunolabeling protocol. The penetration of immunolabels will depend upon the specific type of immunolabel as well as what tissue you are looking at and how it has been prepared. Reach out to us about further extending the penetration of immunolabels into tissues.
| Antibodies | EnCor Cat # | Mouse Brain | Rat Brain | Penetration Depth |
|---|---|---|---|---|
| chicken glial fibrilaral acdic protein | CPCA-GFAP | √ | √ | >600 um (rat) |
| chicken calbindin | CPCA-Calb | √ | √ | > 550 um (rat/mouse) |
| chicken calretinin | CPCA-Calretinin | √ | √ | > 600 um (rat) |
| chicken myelin basic protein | CPCA-MBP | √ | √ | < 300 um (rat) |
| chicken neurofilament-heavy chain | CPCA-NFH | √ | √ | < 300 um (rat) |
| chicken vimentin | CPCA-VIM | √ | √ | < 150 um (rat/mouse) |
| mouse Fox3/NeuN | MCA-1B7 | √ | √ | > 600 um (rat) |
| mouse glial fibrilaral acidic protein | MCA-5C10 | √ | √ | < 300 um (rat) |
| mouse myelin basic protein | MCA-7G7 | √ | √ | < 400 um (rat) |
| mouse neurofilament-heavy chain | MCA-AH1 | √ | √ | < 50 um (rat) |
| mouse vimentin | MCA-2D1 | √ | √ | < 430 um (rat) |
| rabbit cFos | RPCA-cFos | √ | √ | > 500 um (rat) |
| rabbit Fox3/NeuN | RPCA-Fox3 | √ | √ | > 600 (rat) |
| rabbit methy CpG-Binding Protein 2 | RPCA-MeCP2 | √ | √ | > 550 um (rat/mouse) |
| rabbit neurofilament-medium chain | RCPA-NFM | √ | √ | < 200 um (rat/mouse) |
Mouse Brain 20X, 5C10 (red‐546 )/chMBP (green‐488)/Dapi (blue‐405), 600 um
Rat Brain 20X, 5C10 (red‐546 )/chMBP (green‐488)/Dapi (blue‐405), 600 um
Rat Brain 20X, Rab‐cFos (red‐546 )/mo NFH‐AH1 (green‐488)/Dapi (blue‐405), 50 um