Rapid Tissue Clearing


One of the most important attributes of Visikol® HISTO™ is that it rapidly renders biological tissues transparent for subsequent visualization. By allowing for rapid clearing, a researcher can quickly determine if Visikol HISTO meets their bio-imaging needs. 

4mm mouse brain hemisphere clearing in 5 and a half hours. 

Human placenta tissue clearing over a period of 60 minutes. 


Tissue Clearing and Labeling Time Overiew


Thickness Permeabilization and dehydration Penetration / Permeabilization Blocking Antibody Incubation Washing steps HISTO-1 and HISTO-2 or Visikol HISTO-M
8 mm (e.g. whole mouse brain) 2 hr 4 hr 2.5 days 120 hrs 2 hr + overnight for last wash 24 hr
4 mm (e.g. mouse brain hemisphere) 1 hr 3 hr 40 hr 40 hrs 1 hr + overnight for last wash 18 hr
2 mm 45 min 2 hr 13.5 hr 13.5 hrs 45 min 6 hr
1 mm 20 min 1 hr 4.5 hr 4.5 hrs 30 min 2 hr
500 um 8 min 30 min 90 min 90 min 20 min 15 min
≤ 250 um 4 min 15 min 30 min 45 min 10 min 5 min
3D cell culture models (organoids, spheroids, microtissues) 2 min 15 min 15 min 30 min 5 min 2 min