3D cell culture models are increasingly being implemented in high-throughput drug screening due to the higher degree to which they recapitulate critical features of the tumor microenvironment. Particularly in the context of targeted chemotherapeutics, cell-ECM contacts in 3D culture may be crucial to the expression of valuable drug targets, such as estrogen receptor (ER) in the context of breast cancer. At Visikol, we leverage 3D cell culture models to provide advanced in vitro drug discovery services to our clients.
Improved In Vivo Relevancy
Not only are 3D cell culture models known to more accurately represent important physical parameters of the in vivo microenvironment (e.g. transport processes, oxygen and nutrient gradients), but we have shown that these models may offer a better representation of in vivo gene expression. This allows our clients to make more informed decisions regarding the in vivo translatability of targeted thereapeutic screening results, thus reducing costs and increasing the likelihood of success within the drug discovery process.
Optimizing your 3D model
With in-house cell culture capabilities, we offer customized development of 3D culture models using your specific tumor cell lines. Depending on your needs, we can optimize culture conditions to achieve spheroidal morphology and maximal cell viability.
Typically, our oncology drug discovery clients are most interested in understanding how their therapeutic candidate affects cell viability and proliferation. However, assays are customizable to fit your needs.
Quantitative, high-content results
Using customized, in-house image processing algorithms, we can provide digestible insights into your high content screening results. We’ve previously used such quantitative approaches to demonstrate the difference that target expression in 3D culture can make in the context of breast cancer cell sensitivity to the estrogen receptor-targeting drug, fulvestrant. Contact us to find out more about how culture dimensionality might affect expression of your drug target or to discuss how our high-throughput quantitative approaches can help you meet your drug screening needs.