Refractive Index: 1.56 [1]
Clearing Time: Rapid – hours to days [1]
Type: Non-aqueous
IHC: Compatible with immunostaining [1]
FP: Yes, but quenching [1]
Tissue Morphology: Tissue shrinkage[1]
Carcinogenicity: Yes [1]
Difficulty: Easy-to-use
Solvent based clearing with BABB has been used for decades but it is unable to clear myelinated tissues and quickly quenches fluorescent protein (FP) [1],[2]. To combat this problem, Erturk et al. developed 3DISCO which utilizes tetrahydrofuran (THF) instead of alcohol, dibenzyl ether (DBE) and sometimes dichloromethane (DCM) depending upon the tissue [1]. Reiner et al. further developed the 3DISCO technique to improve immunolabeling and this process is referred to as iDISCO. However, both techniques employ the same optical clearing approach and we refer to this DBE-based technique as 3DISCO.
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3DISCO/iDISCO Publications
- Optimizing probes to image cleared tissue
- Combined 3DISCO clearing method, retrograde tracer and ultramicroscopy to map corneal neurons in a whole adult mouse trigeminal ganglion
- High-resolution imaging of entire organs by 3-dimensional imaging of solvent cleared organs (3DISCO)
- Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
- Three-dimensional imaging of solvent-cleared organs using 3DISCO
- iDISCO Info
- Light microscopy mapping of connections in the intact brain
- Clearing and Labeling Techniques for Large-Scale Biological Tissues