Tissue Clearing with BABBMike Johnson2019-09-14T18:15:55-05:00
Refractive Index: 1.55 
Clearing Time: Rapid – days 
Type: Non-aqueous 
IHC: Compatible with immunohistochemistry 
FP: Quenches fluorescent protein 
Tissue Morphology: Tissue shrinkage 
Carcinogenicity: Yes 
Difficulty: Easy-to-use 
BABB is an abbreviation for Benzyl Alcohol/ Benzyl Benzoate whereas the solution is comprised of two parts benzyl benzoate and one part benzyl alcohol . BABB has been used as an optical clearing agent for decades and works very well for many applications such as the clearing of bones . BABB is compatible with immunolabeling and clears tissues quickly, but quenches fluorescent proteins.
While BABB is a very rapid tissue clearing technique, it will cause tissues to shrink and can damage microscope lenses. Leica has though developed a 20x objective specifically for use with BABB (Leica BABB Objective). Tissues that are been left in BABB for too long will eventually become brittle and fall apart.
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