Matching you refractive index
Zeiss suggests reducing the high RI (1.50 to 1.56) of solvent based techniques by using a series of ethanol/DBE treatments after tissue clearing. However, this will likely cause your nuclear stains to come out of your tissue. Additionally, this adds one more variable to your optimization/imaging work and we suggest instead to first image your tissue at their original RI. If you then need to reduce the RI of the tissue to improve image quality we would suggest then using the ethanol/DBE treatments. However, this might cause your tissue to become more opaque.
**Disclaimer – Please contact your product representative from Zeiss or imaging core manager about solvent compatibility prior to imaging tissues with Visikol HISTO**