Multiplexed imaging is a powerful tool that allows researchers to visualize changes in several compartments of the cell with organelle-specific stains. This technique enables researchers to examine changes in cellular pathways with subcellular resolution. In this blog post, we’ll explore how Visikol’s Cell Painting Assay can provide a comprehensive view of cellular pathways, allowing researchers to gain a deeper understanding of how drugs impact cellular processes. From understanding physiological, metabolic, or epigenetic perturbations to analyzing hundreds to thousands of parameters, we’ll explain how Visikol’s Cell Painting Assay can help researchers make informed decisions about which compounds to pursue.
Drug discovery and development is a complex process that involves identifying and testing compounds that can be used to treat diseases. The process can be time-consuming and expensive, and many compounds fail to make it to market. One of the challenges of drug discovery is understanding how drugs interact with cellular pathways. Cellular pathways are complex networks of biochemical reactions that are involved in many physiological processes, including cell growth, differentiation, and death. Understanding how drugs impact cellular pathways is critical to developing effective therapies.
Multiplexed imaging is a technique that allows researchers to visualize multiple markers or stains on a single slide with subcellular resolution. This technique enables researchers to examine changes in several compartments of the cell with organelle-specific stains. The Cell Painting Assay is a high-content, multiplexed image-based assay that utilizes organelle-specific stains to visualize physiological, metabolic, or epigenetic perturbations in different cellular compartments. The assay provides a comprehensive view of cellular pathways by analyzing hundreds to thousands of parameters.
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Through the Cell Painting Assay, researchers can gain a deeper understanding of how drugs impact cellular processes by providing a detailed report that allows clients to explore their specific research questions. The assay can be used to understand physiological, metabolic, or epigenetic perturbations by visualizing changes in different cellular compartments. For example, Visikol scientists used the Cell Painting Assay to elucidate the effects of a small molecule anti-proliferative therapeutic and cytochalasin B in cancer cells. The resulting images showed the effects of the drugs on the ER network, cytoskeleton, and mitochondria. This information can be used to develop a better understanding of how the drugs are working and to optimize their use in drug discovery. The Cell Painting Assay is a powerful tool for drug discovery and development. The assay can be used to identify compounds that affect specific cellular pathways, allowing researchers to make informed decisions about which compounds to pursue. The assay can also be used to optimize the use of existing compounds by providing a better understanding of how they work. By using Visikol’s Cell Painting Assay, researchers can “fail faster” by being able to visualize tangible changes at the cellular level.
Visikol’s Cell Painting Assay is a powerful tool for drug discovery and development. The assay provides a comprehensive view of cellular pathways by analyzing hundreds to thousands of parameters. By using the assay, researchers can gain a deeper understanding of how drugs impact cellular processes, allowing them to make informed decisions about which compounds to pursue. Visikol’s Cell Painting Assay is a valuable tool for pharmaceutical companies, pharmaceutical scientists, biochemists, medicinal chemists, pharmacologists, and toxicologists who are involved in drug discovery and development.
To learn more about the Cell Painting Assay, please reach out to a member of our team.