Protocol for Reversing Clearing of Visikol® HISTO™
In developing Visikol® HISTO™, our most important goal was to preserve the histological integrity of cleared specimens so that clearing can be reversed. The reversibility of Visikol® HISTO™ means you can validate 3D data against slide-based histopathology
The process of reversing Visikol® HISTO™ is rapid and simple:
This protocol is designed to be conducted following clearing and imaging in Visikol® HISTO-1™ or Visikol® HISTO-2™
1. Cleared tissue should be placed directly into a large volume of ethanol (absolute, or histological grade) or methanol. Volume of alcohol should be at least 10-20x tissue volume. Leave tissue at room temperature until opacity has been restored.
2. Larger and more vascular tissues (e.g. whole kidney) may require 2-3 washes of alcohol over the course of several hours.
3. After reversal, samples can be processed directly for paraffin embedding histological preparations