HUREL® Cat™2021-10-20T14:23:18-05:00

HUREL® Cat™

Co-culture comprised of cryopreserved primary feline hepatocytes cultured with cells of non-parenchymal, stromal type.

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Example Metabolic Activity (nmoles/hr/106 cells)

SubstrateEnzymeConcentration (µM)Day 1Day 4Day 8
7-EthoxycoumarinPhase I1000.1100.1170.059
7-HydroxycoumarinPhase II1005.0603.9504.277
7-HydroxycoumarinPhase III1003.6873.2504.090

Culture Condition and Morphology

Cryopreserved primary feline hepatocytes were thawed and plated with HUREL PlatinumHeps™ Media supplemented with 10% serum and subsequently changed 24 hours post-seeding to HUREL PlatinumHeps™ Basal Media. CYP substrate concentrations are given in the table above along with metabolite formation recorded as nmoles/hr/106 cells. All incubations were carried out in triplicate on days 1, 4, and 8 upon cell delivery and incubated for 60 minutes. Reactions took place in a humidified incubator at 37°C, in 5% CO2. Col­lected supernatants were stored at -20°C until further LC/MS/MS analysis.

Day 1 – Morphology

Day-1-cat

Phase contrast image in a 24-well at a 10x magnification.

Day 7 – Morphology

Phase contrast image in a 24-well at a 10x magnification.

Day 7 – Bile Canaliculi

Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (C-DCFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 96-well at 10x magnification with filters EX/EM 492-495/512-527 nm.

Example Culture Origin

Animal Donor Demographics
  • Strain: Feline
  • Number of Donors: 1
  • Age (yrs): –
  • Sex: Male
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