Visikol® for Plant Biology™ was developed as a direct replacement for chloral hydrate for use in botanical microscopy. Chloral hydrate is one of the first tissue clearing techniques and has been used for over a century to render plant tissues transparent. However, it is a controlled narcotic and thus challenging for many researchers to obtain. Below, we provide the protocol for how to use Visikol for Plant Biology to render your plant sample transparent for microscopic visualization.
Visualization of xylem in the developing roots of Arabidopsis thaliana
- Freshly cultured Arabidopsis thaliana plantlets were placed in a 2 dram vial and covered with approximately 5 mL Visikol for Plant Biology.
- The plantlets were let to sit in Visikol for Plant Biology until clear (approximately 1 hour)
- Plantlets were removed and placed on a microscope slide, covered with several drops of Visikol for Plant Biology, and viewed on a Nikon Eclipse 80i microscope, with NIS-Elements D 3.00 SP7 imaging software.
- Added 3 drops toluidine blue to microscope slide, after 10 minutes, re-visualized on microscope.