HUREL® Rabbit™2021-10-20T14:19:06-05:00

HUREL® Rabbit™

Co-culture comprised of cryopreserved primary Rabbit hepatocytes cultured with cells of non-parenchymal, stromal type.

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Example Metabolic Activity (nmoles/hr/106 cells)

Substrate

Enzyme

Concentration (µM)

Day 1

Day 4

Day 8

7-EthoxycoumarinPhase I (ECOD)1003.352.74.94
7-HydroxycoumarinPhase II (UGT)100295.33278.50265.33
7-HydroxycoumarinPhase II(Sult)10048.1737.8330.65

Culture Condition and Morphology

Cryopreserved rabbit hepatocytes were thawed and plated with HUREL PlatinumHeps™ Media supplemented with 10% serum and subsequently changed 24 hours post-seeding to HUREL PlatinumHeps™ maintenance Media. CYP substrate concentrations are given in the table above along with metabolite formation recorded as nmoles/hr/million cells. All incubations were carried out in triplicate on days 1, 4, and 8 (customer day) upon cell delivery and incubated for 60 minutes. Reactions took place in a humidified incubator at 37°C, in 5% CO2. Collected supernatants were stored at -20°C until further LC/MS/MS analysis.

Day 1 – Morphology

rabbit_adobespark (1)

Phase contrast image in a 24-well at a 10x magnification

Day 7 – Morphology

Phase contrast image in a 24-well at a 10x magnification

Day 7 – Bile Canaliculi

rabbit3_adobespark

Bile canaliculi assayed via 5-(and-6)-carboxy-2’, 7’-dichlorofluorescein diacetate (CDFDA) stain at a concentration of 5 µM and imaged in the GFP channel in a 24-well at 10x magnification

Example Culture Origin

Animal Donor Demographics
  • Strain: New Zealand White
  • Number of Donors: 4
  • Age: –
  • Gender: Male
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