Introducing Visikol HISTO-M

Seeing Deeper into 3D Tissue Cultures

Microtissues and organoids are too thick to be fully imaged using confocal microscopy. In PBS, only the outer layers of cells may be imaged.  After treatment with Visikol HISTO-M, cells can be imaged on every plane through the center, allowing you to extract more information from your tissue models.

3D Tissue Culture in PBS

Figure 1. Confocal z-stack montage of NCI-2170 Organoids stained with SYTOX green, in PBS. Notice that only outer layer of cells is visible to imaging. 20 um z-step.

Figure 1. Confocal z-stack montage of NCI-2170 Organoids stained with SYTOX green, in PBS. Notice that only outer layer of cells is visible to imaging. 20 um z-step.

3D Tissue Culture with Visikol HISTO-M

Figure 2. Confocal z-stack montage of Visikol MICRO-treated NCI-2170 organoids stained with SYTOX green. The full plane of cells is visible in each image. 20 um z-step.

Figure 2. Confocal z-stack montage of Visikol MICRO-treated NCI-2170 organoids stained with SYTOX green. The full plane of cells is visible in each image. 20 um z-step.