The performance of Visikol was evaluated in comparison to Chloral Hydrate for several botanical specimens, and Visikol was demonstrated to be effective in clearing a number of botanical species, including Arabidopsis thaliana, Origanum vulgaris, Ocimum americanum, and Zingiber officianale. This work has been published by scientists at Rutgers University and CUNY-BMCC in a peer reviewed scientific article in May's issue of Applications in Plant Sciences, an open access journal published by the Botanical Society of America.
Thomas S. Villani, Adolfina R. Koroch, and James E. Simon
- Premise of the study: This study presents Visikol™ (a new proprietary formulation) which can be used as an efficient replacement for chloral hydrate as a clearing agent for microscopic examination. In the US, chloral hydrate is regulated and therefore difficult to acquire.
- Methods and results: Fresh and dry samples of the following plants: ginger (Zingiber officinale), maté (Ilex paraguariensis), lime basil (Ocimum americanum), oregano (Origanum vulgare) and mouse-ear cress (Arabidopsis thaliana), were cleared using Visikol™ or chloral hydrate solution and compared using a light microscope.
- Conclusions: This new method can be used successfully to clear specimens, allowing for identification of diagnostic characteristics for the identification of plant materials. Visikol™ is as effective as chloral hydrate in providing clarity and resolution of all tissues examined. Tissues become transparent allowing observation of deeper layers of cells making it effective in research, botanical and quality control, and for educational applications.»