OUR PRODUCT AT A GLANCE
HUREL® micro livers are self-assembling co-cultures (SACCs) of primary hepatocytes that are cryopreserved and combined with a non-parenchymal (stromal) cell line. The hepatocytes spontaneously self-assemble into colonies. This patented form of liver cell culture has been demonstrated, in peer-reviewed studies, to produce the longest-enduring, most phenotypically stable and metabolically competent of any liver tissue construct developed in the world to date.
The HUREL Micro Liver’s superior metabolic competency, derived in part from their multi-fold greater hepatocyte number and therefore greater cell density within the microtiter well, is the characteristic most centrally responsible for HUREL’s delivering data of superior translational predictivity for DMPK studies.
Rock-solid, long-enduring phenotypic stability creates the foundation for the superior metabolic competency that distinguishes data generated on the HUREL platform from that of primary hepatocytes cultured in suspension, in monoculture, in micropatterned arrays, and in 3D spheroids.
HUREL Liver Model Species
WHAT’S INCLUDED
Each package will arrive with the following items inside:
-Plated Cells
-Dosing & Maintenance Media
-Spare Lids
-Heat Packs
-Temperature Log
-Usage Instructions
OUR PACKAGING
To ensure products are delivered to our clients unharmed, we have developed a robust packaging method designed with viability in mind.
Orders will arrive in signature Visikol packaging, complete with an inner and outer Styrofoam cooler within.
Inner cooler arrives safely and securely nestled between two Styrofoam spacers.
Media, plate lids, and cells can be found within the inner cooler.
Usage Protocols
HUREL® hepatic co-culture plates are shipped in a uniquely designed configuration to ensure temperature control and leakage prevention for the duration of shipment. Kindly follow our unpacking protocol for optimal use of our product.
Package Contents:
- HUREL maintenance media (serum-containing media): 65ml per plate
- The serum-containing maintenance media is recommended to use for media change upon plate arrival and maintaining the culture plates (change media every 2 days) prior to assays. For long-term assays (> 7 day culture), the maintenance media is recommended to use for cell stability.
- HUREL dosing media (serum-free media): 65ml per plate
- The serum-free dosing media is recommended to use for assays that take up to 7 days. For drug clearance and metabolite generation assays, the media don’t need to be changed up to 7 days.
- HUREL® co-culture plates, Replacement lids, Unpacking & Maintenance Protocol, COA lot information, Packing slip
- Temperature recorder* (disposable): 1) Connect the temp recorder to PC USB port, 2) Go to the USB drive and find the pdf output file, 3) Email the pdf output file to cheul.cho@visikol.com
*No software required. The temp. recorder will generate pdf output file automatically in the USB drive once connected
Box unpacking
1.Take both medium bottles from shipping container and warm the medium bottle labeled Maintenance Media to 37°C
2. Carefully remove HUREL® hepatic co-culture plates from shipping container
Keep cell plate orientation as it is packed. DO NOT flip over plates
3. Transfer cell plates to biosafety cabinet
Only use cells under aseptic conditions
4. Follow “Unpacking HUREL® plate” (see below)
5. Remove the lid and peel off the sealing film
6. Aspirate the shipping media from the plates using the aspiration protocol detailed below and replace with 37°C warm Maintenance Media. Refer to the table “Incubation volume” below for recommended medium volume/well
7. Finally, put a sterile replacement lid on the co-culture plate and transfer to an incubator for incubation at 37°C and 5% CO2**
** Visikol recommends that the HUREL plates acclimate in an incubator overnight, however, for same day experiments, allow the cells to acclimate in an incubator for a minimum of at least 4 hours before use in an experiment. It is recommended to use the culture plate within 4 days of receiving and to change the plate’s media with the maintenance media every 2 days.
Recommended Aspiration Protocol
- Medium aspiration should be performed using a low vacuum pressure
- Aspirator tips should NEVER touch the bottom of the wells
- To ensure cells do not dry out and function and viability are sustained, it is recommended to aspirate and replace medium 3-6 columns at a time
Unpacking HUREL Plate:
- Open the cell culture plate from the sleeve and remove the rubber bands
- Remove the lid
- Peel off the sealing film
Incubation Volume
HUREL Human Pool™/ HUREL Human™/ HUREL Primate™/HUREL Rabbit™/ HUREL Cat™
Plate Format | Hepatocyte Number/Well | Recommended |
|---|---|---|
| 384-well | 11,000 | 30-50 μL |
| 96-well | 30,000 | 80-100 μL |
| 48-well | 70,000 | 180-200 μL |
| 24-well | 188,000 | 400-500 μL |
| 12-well | 375,500 | 800-1000 μL |
| 6-well | 940,000 | 2000-2400 μL |
HUREL Dog™
Plate Format | Hepatocyte Number/Well | Recommended |
|---|---|---|
| 384-well | 15,000 | 30-50 μL |
| 96-well | 40,000 | 80-100 μL |
| 48-well | 94,000 | 180-200 μL |
| 24-well | 250,000 | 400-500 μL |
| 12-well | 500,000 | 800-1000 μL |
| 6-well | 1,250,000 | 2000-2400 μL |
HUREL Rat SD™/ HUREL Rat WH™/ HUREL Minipig™
Plate Format | Hepatocyte Number/Well | Recommended |
|---|---|---|
| 384-well | 8,000 | 30-50 μL |
| 96-well | 25,000 | 80-100 μL |
| 48-well | 59,000 | 180-200 μL |
| 24-well | 156,000 | 400-500 μL |
| 12-well | 312,500 | 800-1000 μL |
| 6-well | 780,000 | 2000-2400 μL |
HUREL Mouse™
Plate Format | Hepatocyte Number/Well | Recommended |
|---|---|---|
| 384-well | 7,000 | 30-50 μL |
| 96-well | 20,000 | 80-100 μL |
| 48-well | 47,000 | 180-200 μL |
| 24-well | 125,000 | 400-500 μL |
| 12-well | 250,000 | 800-1000 μL |
| 6-well | 625,000 | 2000-2400 μL |
