Below is a non-comprehensive list of antibodies that we have validated for use with Visikol® HISTO™ tissue clearing. The specific depth of penetration for each antibody will be dependent upon tissue type, dilution as well as incubation time. We always suggest optimizing your antibody in small sections and working your way up to your desired thickness as you optimize dilution and incubation time. 

Ms = mouse, Rt = rat, Hu = human, Nhp = non-human primate, Ca = Canine

ANTIGEN HOST SPECIES TARGET SPECIES SUPPLIER CAT #
ACTIN mouse human, mouse, rat, rabbit, canine, chicken Invitrogen MA511869
ADENYLATE CYCLASE III Rabbit Ms, Rt EnCor Bio RPCA-ACIII
BETA
CATENIN
Rabbit (monoclonal) Ms, Hu AbCam ab32572
BETA
INTEGRIN
Rabbit (monoclonal) Ms, Hu AbCam ab52971
CALBINDIN Chicken Ms,Rt, Hu EnCor Bio CPCA-Calb
CALRETNIN Chicken Ms,Rt, Hu EnCor Bio CPCA-Calretinin
CD68 Mouse Hu Invitrogen 14068980
CFOS Rabbit Ms, Rt, Hu EnCor Bio RPCA-c-Fos-AP
CK-14 mouse Hu, Rt Invitrogen MA511599
COLLAGEN IV goat Hu Invitrogen PA136001
E-CADHERIN Rabbit (monoclonal) Ms, Hu AbCam ab40772
E-CADHERIN Rat Hu Invitrogen 53324982
EGFR Rabbit Hu, Ms, Rt, Nhp Invitrogen PA1-1110
ERBB2 (HER2) rabbit Hu, Ca Invitrogen PA514635
FOX3/NEUN Mouse (monoclonal) Ms, Rt, Hu, all vertebrates EnCor Bio MCA-1B7
FOX3/NEUN Rabbit Ms, Hu, vertebrates EnCor Bio RPCA-Fox3
GFAP chicken Hu, Ms, Rt Encore CPCA-GFAP
GFAP rabbit Hu, Ms, Rt Encore RPCA-GFAP
GFAP Chicken Ms,Rt, Hu EnCor Bio CPCA-GFAP
GFAP Mouse (monoclonal) Ms, Rt, Hu, all mammals EnCor Bio MCA-5C10
KI67 Rabbit Ms, Hu AbCam ab15580
KI67 Rabbit (monoclonal) Ms, Hu AbCam av16667
KI67 rabbit Hu, Rt Thermo Scientific Lab Vision RB1510
METHY
CPG-BINDING PROTEIN 2
Rabbit Ms, Hu EnCor Bio RPCA-MeCP2
MYELIN
BASIC PROTEIN
Chicken Ms,Rt, Hu EnCor Bio CPCA-MBP
MYELIN
BASIC PROTEIN
Mouse (monoclonal) Ms, Rt, Hu, all mammals EnCor Bio MCA-7G7
NEUN-ALEXAFLUOR488
CONJUGATE
Rabbit Ms, Rt Millipore ABN-90
NEUROFILAMENT-HEAVY
CHAIN
Mouse (monoclonal) Ms, Rt, Hu, all mammals EnCor Bio MCA-AH1
NEUROFILAMENT-MEDIUM
CHAIN
Rabbit Ms EnCor Bio RCPA-NF-M
NEUROFILAMNET-HEAVY
CHAIN
Chicken Ms,Rt, Hu EnCor Bio CPCA-NFH
PAN-CYTOKERATIN ALEXA-488 Mouse Hu, Rt, Ms, Nhp Invitrogen 53900380
PARVALBUMIN Mouse (monoclonal) Hu, Ms, Rt Swant PV-235
PDGF-BETA Rabbit Hu Invitrogen PA511723
PDX-1 guinea pig Mt, Hu Abcam 47308
PRO-CASPASE 3 mouse Hu Invitrogen MA141163
VIMENTIN Chicken Ms,Rt, Hu EnCor Bio CPCA-Vime
VIMENTIN Mouse (monoclonal) Ms, Rt, Hu, all mammals EnCor Bio MCA-2D1
VIMENTIN Rabbit Hu, Ms, Rt Invitrogen PA527231