Low RNA Concentration in qPCR: Challenges and Solutions
Quantitative Polymerase Chain Reaction (qPCR) is a widely used molecular biology technique for quantifying gene expression levels. However, performing [...]
Understanding Liver Fibrosis and the Role of In Vitro Models
Liver fibrosis is a serious condition that affects millions of people worldwide. It is a pathological overaccumulation of excess [...]
What are the Differences Between qPCR and RT-PCR?
The polymerase chain reaction (PCR) is a powerful molecular biology technology that is used to recognize a specific DNA [...]
Making Sense of ΔΔCT and Fold Change Values
Quantitative PCR (qPCR) is a powerful tool for analyzing gene expression in all sorts of biological samples, but its [...]
An Overview of Complementary DNA Synthesis
Many biological processes, such as gene cloning and gene expression analysis using real-time polymerase chain reaction (qPCR) and high-throughput [...]
qPCR for Assays Other Than Gene Expression
While quantitative polymerase chain reaction, commonly known as real-time PCR or simply as qPCR, is often employed for studies [...]
Spinning Lots of Plates
PCR (Polymerase Chain Reaction) is a notoriously finicky operation. While current reagent technologies have made it a very robust [...]
Examining gene expression with RT-qPCR
At Visikol, our services have grown from our expertise in cell culture, high-content tissue imaging, and advanced digital pathology. [...]