Understanding Blocking Buffers in Immunofluorescence Workflows
Reducing the nonspecific binding of secondary antibodies in immunofluorescence workflows is critical to generating high quality data from tissues [...]
The Importance of Dehydration and Rehydration of Fixed Tissue for Immunolabeling
In order to optimize the quality of microscopic images obtained from Formalin Fixed Paraffin Embedded (FFPE) samples, the starting [...]
How Image Filtering Works
When analyzing images, filters are essential for pre-processing images to make them easier to interpret and analyze. At Visikol, [...]
Our frenemies, RNases
Over the past few decades, the popular scientific view of ribonucleases – aka RNases – has become much richer [...]
Gene-specific methylation analysis by LC-MS/MS
DNA methylation is a critical epigenetic modification that controls gene expression and genome stability, which has oncogenic implications during [...]
Combining Cell Painting and Antibody Labeling
The cell painting assay is a high-content image-based method for morphological profiling using fluorescent dyes. These dyes reveal relevant [...]
What is Image Analysis?
When it comes to biomedical research, quantitative data is always preferred to qualitative data since it is measurable and [...]
Causes of Autofluorescence
One of Visikol's main areas of focus is histology and multiplex imaging. Visikol’s multiplex imaging is driven by patented [...]
Researchers Use HISTO to Study Reginal Ganglion Cells
While axons in the peripheral nerve tend to regenerate well, central nervous system axons have issues regenerating post-trauma. In [...]
Working with Spheroids: Challenges, Limitations and Effectiveness
When working on drug discovery and assay development, scientists often use models that can help mimic the effects of [...]