Easily validate your 3D data against traditional 2D histology

Visikol HISTO Reversal Paradigm

Visikol HISTO preserves tissues!  Tissue clearing techniques like CLARITY, SeeDB, BABB and 3DISCO irreversibly change the cellular structure of tissues, since they rely on removal of lipid structures and/or hydration of protein structure.

Say "so long" to stereology!  With Visikol HISTO, traditional 2D H&E processing can be completed after 3D imaging, so you can choose exactly which sections are of interest, before the tissue is even embedded.  

Obtain 3D image stacks and 2D H&E slides from the same sample!  You don't have to choose between 3D imaging and your conventional tissue-imaging workflow, Visikol fits right in so you can get the most out of each specimen! 

H&E sections after clearing with Visikol HISTO

Brain tissue (mouse) - H&E and GFAP-labeled IHC sections of mouse brain tissue after clearing with Visikol HISTO

Kidney (mouse) - H&E section of mouse kidney following clearing and reversal. 

Lung (mouse) - H&E section of mouse lung following clearing and reversal. 

Liver (mouse) - H&E section of a mouse liver following clearing and reversal. 

Placenta tissue (human) - H&E sections of human placenta tissue after clearing reversal.