Multiplex tissue imaging refers to the concept of applying multiplex markers (typically >3) to a single tissue so that many biomarkers can be visualized together. Traditionally, chromogenic IHC is limited to 2-3 markers as overlapping colors prevent effective deconvolution. For many applications, only 2-3 markers is acceptable for addressing a research question but it is highly limited for addressing complex research questions. For example, in the context of immunology and immuno-oncology it is critically important to understand the complex interplay between different types of immune cells and the underlying pathology. This complexity necessitates multiple tissue labels where many markers can be visualized simultaneously.
If you are interested in learning more about multiplex tissue imaging check out the resources below or reach out to our team to discuss your next project: